C. Allex, S. Baldwin, J. Shavlik & F. Blattner (1997).
Increasing Consensus Accuracy in DNA Fragment Assemblies by Incorporating Fluorescent Trace Representations. Proceedings, Fifth International Conference on Intelligent Systems for Molecular Biology, pp. , Halkidiki, Greece. AAAI Press.

This publication is available in PDF and available in postscript.

Abstract:

We present a new method for determining the consensus sequence in DNA fragment assemblies. The new method, Trace-Evidence, directly incorporates aligned ABI trace information into consensus calculations via our previously described representation, Trace-Data Classifications. The new method extracts and sums evidence indicated by the representation to determine consensus calls. Using the Trace-Evidence method results in automatically produced consensus sequences that are more accurate and less ambiguous than those produced with standard majority-voting methods. Additionally, these improvements are achieved with less coverage than required by the standard methods using Trace-Evidence and a coverage of only three, error rates are as low as those with a coverage of over ten sequences.

Return to the publications of the Univ. of Wisconsin Machine Learning Research Group.