GreenNP is a database search engine to query neuropeptides in green crab.

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Usage of GreenNP
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1. User first needs to download GreenNPDB database and save it in a local computer.
2. Download GreenNP search engine.
3. Start GreenNP (windows-based GUI)

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Information about query
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User can query in the following fields: Mass(accuracy), family (name), sequence, PTM, FT-MS, QTOF, Brain, PO, TG, SG, STG.

Mass: 
the molecular weight of a sequence, Da.

Family(name): 
AST-A
AST-B
Bursicon a
Bursicon b
CCAP
CCAP precursor-related peptide
CHH
CHH precursor-Related Peptide (CPRP)
Corazonin
FLP
HIGSLYRa
HIGSLYRa precursor-related peptide
MIH
Neuroparsin
Orcokinin
Orcomyotropin
Others
PDH precursor-related peptide
PDH
Proctolin
AKH/RPCH
AKH/RPCH precursor-related peptide
RYamide
SIFamide
Tachykinin-like

Sequence:
no limit on length

PTM:
N-terminal amidation, Oxidation of methionine, pyroglutamate, none

FT-MS, QTOF, Brain, PO, TG, SG, STG:
+,-

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Output
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Search resule will display in the bottom window, user will copy (right click mouse to find"copy") them to clipboard for further analysis. 



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Contact information
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Weifneg Cao
wcao2@wisc.edu


All rights reserved. 
Li lab
University of Wisconsin-Madison
May 22,2011