no other articles link to it . please introduce links to this page from related articles ; try the find link tool for suggestions . september 2013 bia separations type private industry high performance liquid chromatography biotechnology biochromatography founded ljubljana slovenia 1998 headquarters villach austria area served worldwide products monolithic hplc columns subsidiaries slovenia united states china website biaseparations.com bia separations is a biotechnology company focused on the production of methacrylate monolithic hplc columns and developing industrial purification processes and analytical methods . contents 1 company history 2 technology 3 applications 4 the monolith summer school 5 references 6 external links company history edit bia separations was formed in 1998 by a group of scientists in ljubljana slovenia to commercialize a new chromatographic resin based on monolith technology . monolithic resin was developed independently by three different laboratories in late 1980s led by hjert n vec and tennikova . 1 the company was one of the few slovenian companies to receive venture capital funding at that time horizonte venture management realizing the potential of monoliths made the initial investment in the company . during that year bia separations released its first commercial product marketed under the trade name of cim convective interaction media . the new technique was named hpmc high performance monolith chromatography . during the 1999 and 2000s the company promoted this new platform technology to the scientific and business community and registered several patents . 2 in 2007 the company headquarters moved to villach austria . in 2011 bia separations entered a strategic partnership with japan synthetic rubber corporation a japanese company producing synthetic materials . 3 a strategic partnership agreement with showa denko k.k. sdk was signed in 2012 . 4 technology edit cim monoliths are made of porous methacrylate polymers composed of interconnected channels that range in size from 1 6 m. 5 it is these channels that account for the media separation power and flow characteristics and ability to purify large biomolecules such as large proteins immunoglobulins plasmid dna and viruses with their activity intact . components to be separated are conveyed to the active groups located on the surface of the channels by bulk flow of the mobile phase . since the channels are interconnected there is no diffusion no dead end pores no void volume and no stagnant zones to slow down the transport between the stationary and mobile phase . consequently monolithic columns have flow independent resolution and binding capacity . they exhibit low back pressure even at very high flow rates . in this way the purification time can be significantly decreased resulting in a pronounced reduction of the purification process costs . applications edit depending on the column size cim monolithic columns are primarily used for the purification or analysis of large biomolecules which are being used for cancer gene therapy treatment of viral infectious diseases and treatment of genetic diseases . some types of molecules that can be successfully purified using cim columns are pdna 6 igm 7 inter alpha inhibitors 8 virus like particles 9 and diverse viruses ; adenoviruses 10 bacteriophages 11 feline calicivirus 12 hepatitis a 12 lentivirus 13 influenza a and b 14 rabies virus 15 rotavirus 16 tomato and pepino mosaic virus . 17 the monolith summer school edit since 2004 bia separations has organized and hosted the monolith summer school and symposium mss which takes place every 2 years . mss was established as there were no dedicated conferences to this technology and to bring together the top international scientists and researchers in the area of monolith chromatography to share their experiences and innovative applications . references edit f. vec t. b. tennikova z. deyl monolithic materials preparation properties and applications journal of chromatography library elsevier 2003 cim convective interaction media bia separations a model sme centre for bio separation technology and bia separations cooperate on purification of biomolecules jsr expands its biomedical business by investing in bia separations unique purification technology jsr buys into austrian separations solutions manufacturer sdk invests in biasep to get resins know how cim technology agilent technologies bia separations gmbh sign agreement giving agilent access to bia separations bio monolithic technology shin min jae ; tan lihan ; jeong min ho ; kim ji heung ; choe woo seok 2011 . monolith based immobilized metal affinity chromatography increases production efficiency for plasmid dna purification . journal of chromatography a. 1218 31 5273 5278 . doi 10.1016 j.chroma .2011.06.040 . issn 0021 9673 . smrekar franc ; podgornik ale ; ciringer mateja ; kontrec sandra ; raspor peter ; trancar ale ; peterka matja 2010 . preparation of pharmaceutical grade plasmid dna using methacrylate monolithic columns . vaccine . 28 8 2039 2045 . doi 10.1016 j.vaccine .2009.10.061 . issn 0264 410x . krajnc nika lendero ; smrekar franc ; trancar ale ; podgornik ale 2011 . adsorption behavior of large plasmids on the anion exchange methacrylate monolithic columns . journal of chromatography a. 1218 17 2413 2424 . doi 10.1016 j.chroma .2010.12.058 . issn 0021 9673 . gagnon pete ; hensel frank ; lee soon ; zaidi simin 2011 . chromatographic behavior of igm dna complexes . journal of chromatography a. 1218 17 2405 2412 . doi 10.1016 j.chroma .2010.12.066 . issn 0021 9673 . lim yow pin ; josic djuro ; callanan helen ; brown jeanne ; hixson douglas c. 2005 . affinity purification and enzymatic cleavage of inter alpha inhibitor proteins using antibody and elastase immobilized on cim monolithic disks . journal of chromatography a. 1065 1 39 43 . doi 10.1016 j.chroma .2004.11.006 . issn 0021 9673 . koho tiia ; m ntyl tuomas ; laurinm ki pasi ; huhti leena ; butcher sarah j. ; vesikari timo ; kulomaa markku s. ; hyt nen vesa p. 2012 . purification of norovirus like particles vlps by ion exchange chromatography . journal of virological methods . 181 1 6 11 . doi 10.1016 j.jviromet .2012.01.003 . issn 0166 0934 . urbas lidija ; jarc barbara lah ; barut milo ; zochowska monika ; chroboczek jadwiga ; pihlar boris ; szolajska ewa 2011 . purification of recombinant adenovirus type 3 dodecahedric virus like particles for biomedical applications using short monolithic columns . journal of chromatography a. 1218 17 2451 2459 . doi 10.1016 j.chroma .2011.01.032 . issn 0021 9673 . whitfield robert j. ; battom suzanne e. ; barut milo ; gilham david e. ; ball philip d. 2009 . rapid high performance liquid chromatographic analysis of adenovirus type 5 particles with a prototype anion exchange analytical monolith column . journal of chromatography a. 1216 13 2725 2729 . doi 10.1016 j.chroma .2008.11.010 . issn 0021 9673 . adriaenssens evelien m. ; lehman susan m. ; vandersteegen katrien ; vandenheuvel dieter ; philippe didier l. ; cornelissen anneleen ; clokie martha r.j. ; garc a andr s j. ; de proft maurice ; maes martine ; lavigne rob 2012 . cim monolithic anion exchange chromatography as a useful alternative to cscl gradient purification of bacteriophage particles . virology . 434 2 265 270 . doi 10.1016 j.virol .2012.09.018 . issn 0042 6822 . smrekar franc ; ciringer mateja ; trancar ale ; podgornik ale 2011 . characterisation of methacrylate monoliths for bacteriophage purification . journal of chromatography a. 1218 17 2438 2444 . doi 10.1016 j.chroma .2010.12.083 . issn 0021 9673 . kramberger petra ; honour richard c. ; herman richard e. ; smrekar franci ; peterka matja 2010 . purification of the staphylococcus aureus bacteriophages vdx 10 on methacrylate monoliths . journal of virological methods . 166 1 2 60 64 . doi 10.1016 j.jviromet .2010.02.020 . issn 0166 0934 . a b kova katarina ; guti rrez aguirre ion ; banjac marko ; peterka matja ; polj ak prijatelj mateja ; et al. 2009 . a novel method for concentrating hepatitis a virus and caliciviruses from bottled water . journal of virological methods . 162 1 2 272 275 . doi 10.1016 j.jviromet .2009.07.013 . issn 0166 0934 . bandeira vanessa ; peixoto cristina ; rodrigues ana f. ; cruz pedro e. ; alves paula m. ; coroadinha ana s. ; carrondo manuel j. t. 2012 . downstream processing of lentiviral vectors releasing bottlenecks . human gene therapy methods . 23 4 255 263 . doi 10.1089 hgtb .2012.059 . issn 1946 6536 . industrial platform for virus purification influenza virus purification platform urbas lidija ; ko ir bo tjan ; peterka matja ; pihlar boris ; trancar ale ; barut milo 2011 . reversed phase monolithic analytical columns for the determination of ha1 subunit of influenza virus haemagglutinin . journal of chromatography a. 1218 17 2432 2437 . doi 10.1016 j.chroma .2010.12.082 . issn 0021 9673 . improvement of rabies vaccine production using monolithic chromatographic support guti rrez aguirre ion ; banjac marko ; steyer andrej ; polj ak prijatelj mateja ; peterka matja ; trancar ale ; ravnikar maja 2009 . concentrating rotaviruses from water samples using monolithic chromatographic supports . journal of chromatography a. 1216 13 2700 2704 . doi 10.1016 j.chroma .2008.10.106 . issn 0021 9673 . kramberger petra ; peterka matja ; boben jana ; ravnikar maja ; trancar ale 2007 . short monolithic columns a breakthrough in purification and fast quantification of tomato mosaic virus . journal of chromatography a. 1144 1 143 149 . doi 10.1016 j.chroma .2006.10.055 . issn 0021 9673 . external links edit bia separations home page monolith events retrieved from https en.wikipedia.org w index.php title bia separations & oldid 723664298 categories biotechnologyhidden categories orphaned articles from september 2013all orphaned articles navigation menu personal tools not logged intalkcontributionscreate accountlog in namespaces article talk variants views read edit view history more search navigation main pagecontentsfeatured contentcurrent eventsrandom articledonate to wikipediawikipedia store interaction helpabout wikipediacommunity portalrecent changescontact page tools what links hererelated changesupload filespecial pagespermanent linkpage informationwikidata itemcite this page print export create a bookdownload as pdfprintable version languages add links this page was last modified on 4 june 2016 at 11 55 . text is available under the creative commons attribution sharealike license ; 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