please help improve it or discuss these issues on the talk page . learn how and when to remove these template messages this article is an orphan as no other articles link to it . please introduce links to this page from related articles ; try the find link tool for suggestions . september 2010 this article may require cleanup to meet wikipedia s quality standards . no cleanup reason has been specified . please help improve this article if you can . june 2010 learn how and when to remove this template message learn how and when to remove this template message mirus bio formerly mirus bio corporation develops and manufactures transfection reagents and related products for life science research . history edit mirus corporation was founded in madison wisconsin in 1995 by three university of wisconsin madison scientists jon a. wolff james e. hagstrom and vladimir g. budker . mirus focused on developing innovative non viral gene delivery technologies for gene therapy applications . these innovations also served as the basis for the company s transfection formulations and nucleic acid labeling and conjugation chemistries . mirus researchers published several groundbreaking achievements including the first demonstrations that plasmid dna could be effectively delivered to the rodent liver and skeletal muscle cells using rapid intravascular injections 1 2 ; the first demonstration that caged dna containing nanoparticles are resistant to aggregation under physiologic salt conditions 3 ; the first demonstration of sirna mediated knockdown of an endogenously expressed gene in mice 4 ; development of low toxicity dna containing nanoparticles for gene delivery to the lungs 5 ; development of a clinically viable high efficiency method for delivering plasmid dna into mammalian skeletal muscle 6 ; development of a genetic immunization method for research animals 7 ; development of a technology for targeted delivery of sirna to mouse liver 8 ; new chemistries for transfection 9 ; development of a non viral vector providing sustained liver specific transgene expression for more than one year 10 . in 2008 the therapeutics division was acquired by hoffmann la roche inc. mirus former research tools division remains an independent entity now known as mirus bio llc . references edit 1 . budker v zhang g knechtle s wolff ja . naked dna delivered intraportally expresses efficiently in hepatocytes . gene ther . 1996 jul ; 3 7 593 8 . 2 . budker vg zhang g danko i williams p wolff j. the efficient expression of intravascularly delivered dna in rat muscle . gene ther 1998 5 272 276 . 3 . trubetskoy vs loomis a slattum pm hagstrom je budker vg wolff ja . caged dna does not aggregate in high ionic strength solutions . bioconjug chem . 1999 jul aug ; 10 4 624 8 . 4 . lewis dl hagstrom je loomis ag wolff ja herweijer h. efficient delivery of sirna for inhibition of gene expression in postnatal mice . nat genet . 32 107 2002 . 5 . trubetskoy vs wong sc subbotin v budker vg loomis a hagstrom je wolff ja . recharging cationic dna complexes with highly charged polyanions for in vitro and in vivo gene delivery . gene ther . 2003 feb ; 10 3 261 71 . 6 . hagstrom je hegge j zhang g noble m budker v lewis dl herweijer h wolff ja . a facile nonviral method for delivering genes and sirnas to skeletal muscle of mammalian limbs . mol ther . 2004 aug ; 10 2 386 98 . 7 . bates mk zhang g sebestyen mg neal zc wolff ja herweijer h. genetic immunization for antibody generation in research animals by intravenous delivery of plasmid dna . biotechniques . 2006 feb ; 40 2 199 208 . 8 . rozema db lewis dl wakefield dh wong sc klein jj roesch pl bertin sl reppen tw chu q blokhin av hagstrom je wolff ja . dynamic polyconjugates for targeted in vivo delivery of sirna to hepatocytes . proc natl acad sci usa 7 august 2007 104 32 12982 7 . 9 . wakefield dh klein jj wolff ja rozema db . membrane activity and transfection ability of amphipathic polycations as a function of alkyl group size . bioconjug chem . 2005 sep oct ; 16 5 1204 8 . 10 . wooddell c reppen t wolff ja herweijer h. sustained liver specific transgene expression from the albumin promoter in mice following hydrodynamic plasmid dna delivery . j gene med10 551 2008 . external links edit official website retrieved from https en.wikipedia.org w index.php title mirus bio & oldid 601836830 categories biotechnology companies of the united stateshidden categories orphaned articles from september 2010all orphaned articlesarticles needing cleanup from june 2010all articles needing cleanupcleanup tagged articles without a reason field from june 2010wikipedia pages needing cleanup from june 2010 navigation menu personal tools not logged intalkcontributionscreate accountlog in namespaces article talk variants views read edit view history more search navigation main pagecontentsfeatured contentcurrent eventsrandom articledonate to wikipediawikipedia store interaction helpabout wikipediacommunity portalrecent changescontact page tools what links hererelated changesupload filespecial pagespermanent linkpage informationwikidata itemcite this page print export create a bookdownload as pdfprintable version languages add links this page was last modified on 29 march 2014 at 17 34 . text is available under the creative commons attribution sharealike license ; 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