articles link to it . please introduce links to this page from related articles ; try the find link tool for suggestions . october 2012 flexgen b.v. fast industry biotechnology founded 2004 headquarters leiden netherlands area served worldwide products flexarrayer flexelect custom oligopools services custom oligopool synthesis website http www.flexgen.nl flexgen is a biotechnology company based in leiden netherlands . flexgen is a spin off from leiden university medical centre and dutch space part of eads and has proprietary technologies for laser based in situ synthesis of oligonucleotides and other biomolecules . contents 1 products 1.1 flexelect 1.2 flexarrayer 2 technology 3 references 4 external links products edit flexelect edit flexelecttm oligopools consist of custom oligonucleotides in solution and can be used for in solution target enrichment prior to next generation dna sequencing.target enrichment or in solution hybrid selection is a method for genomic selection in an increasing amount of applications 1 2 such as ; analysis of custom genomic regions of interest e.g. specific genes multiple variants and or complete pathways . analysis of chromosomal translocation validation of single nucleotide polymorphism or snps typically after whole genome or whole exome studies other research and diagnostic applications e.g. synthetic biology an example of a recent application is testing of the brca1 and brca2 breast cancer risk genes 3 flexarrayer edit the flexarrayer is an in house custom oligonucleotide synthesis instrument . the flexarrayer facilitates high troughput synthesis of flexelect oligopools for in solution target enrichment as well as custom microarray production . the flexarrayer is also applicable for array based re sequencing . 4 the flexarrayer provides microarray and oligopool synthesis typically used by genomics centres and sequencing facilities health and safety institutes & microbiology labs technology innovators in the fields of surface chemistries pna s peptide nucleic acid sirna s small interfering rna and more technology edit production of microarrays and flexelecttm oligopools is done with the flexarrayer see image using proprietary technology . the flexarrayer synthesizes custom probesets on a substrate based on oligonucleotide deprotection technology ; before the first oligonucleotide synthesis step the complete dna microarray surface is covered by photolabile groups . those spots the first nucleotide addition is to occur are individually activated by the laser . the nucleotide solution is washed over the microarray surface and the nucleotides chemically bind to the activated spots . all nucleotides contain a photolabile group that can in turn be activated . as many rounds of photoactivation and nucleotide addition are performed as are required to synthesize oligonucleotides of the desired length . this is repeated up to 60 times until the required sequences up to 100.000 have been synthesized . thus the maximum length of any oligonucleotide produced on this platform is 60mer in length . the microarray is now ready to be used alternatively the oligonucleotides can be cleaved off to produce flexelecttm oligopools . the flexarrayer an oligonucleotide synthesis instrument . references edit mamanova l. ; coffey a. j. ; scott c. e. ; kozarewa i. ; turner e. h. ; kumar a. ; howard e. ; shendure j. ; turner d. j. 2010 . target enrichment strategies for next generation sequencing . nature methods . 7 2 111 118 . doi 10.1038 nmeth .1419 . pmid 20111037 . mamanova l. ; coffey a. j. ; scott c. e. ; kozarewa i. ; turner e. h. ; kumar a. ; howard e. ; shendure j. ; turner d. j. 2010 . target enrichment strategies for next generation sequencing . nature methods . 7 6 479 . doi 10.1038 nmeth0610 479c . tewhey r. ; nakano m. ; wang x. ; pab n pe a c. ; novak b. ; giuffre a. ; lin e. ; happe s. ; roberts d. n. ; leproust e. m. ; topol e. j. ; harismendy o. ; frazer k. a. 2009 . enrichment of sequencing targets from the human genome by solution hybridization . genome biology . 10 10 r116 . doi 10.1186 gb 2009 10 10 r116 . pmc 2784331 . pmid 19835619 . two european labs turn to next gen sequencing for brca1 2 testing insequence october 26 2010 . sm carr et al. dna re sequencing with a microarray 2008 . comp biochem physiol d genomics & proteomics 3 1 11 link external links edit flexgen s company website retrieved from https en.wikipedia.org w index.php title flexgen & oldid 678447674 categories biotechnology companies of the netherlandscompanies established in 2004microarrayscompanies based in leidenhidden categories orphaned articles from october 2012all orphaned articlespages using infobox company with unsupported parameters navigation menu personal tools not logged intalkcontributionscreate accountlog in namespaces article talk variants views read edit view history more search navigation main pagecontentsfeatured contentcurrent eventsrandom articledonate to wikipediawikipedia store interaction helpabout wikipediacommunity portalrecent changescontact page tools what links hererelated changesupload filespecial pagespermanent linkpage informationwikidata itemcite this page print export create a bookdownload as pdfprintable version languages add links this page was last modified on 29 august 2015 at 13 05 . text is available under the creative commons attribution sharealike license ; 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