or sign up my account my neb quick order 0 items migrate to monarch migrate to monarch research spotlight hear how neb customers use nebuilder hifi for their dna assembly applications in the latest episode of neb tv . more learn how fsb1 is used as a model to indicate ways in which m13 phage display can be improved by using a system designed to meet the folding requirements of target proteins.more specificity and accuracy of dna ligation depends upon selection of the appropriate ligase and optimization of reaction conditions . reliable single base mismatch discrimination can be achieved.more learn more about the inhibitory effect of non substrate and substrate dna on ligation reactions catalyzed by t4 dna ligase . more learn more about the self labeling halotag protein which can act as a light emitting reporter for protein fusions allowing visualization in fluorescent assays . more behind the paper neb and nibrt scientists devised a robust workflow for comprehensive profiling of gsl head group glycans . chris shares details of this recent publication.more protocols popular all pcr using q5 high fidelity dna polymerase m0491 t7 endonuclease i based mutation detection with the engen mutation detection kit neb e3321 engen sgrna synthesis kit s. pyogenes protocol neb e3322 transfection of cas9 rnp ribonucleoprotein into adherent cells using the lipofectamine rnaimax electroporation of cas9 rnp ribonucleoprotein into adherent cells using the neon electroporation browse all neb protocols we have nearly 1 000 protocols ; care to narrow that list down select a category buffers cellular analysis competent cells discontinued dna assembly cloning and mutagenesis kits dna modifying enzymes & cloning technologies dna plasmids epigenetics gene expression genome editing glycobiology library preparation for next generation sequencing markers & ladders nucleic acid purification pcr polymerases & amplification technologies protein expression & purification technologies protein 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mutagenesis kitsgenome editingcompetent cellsmarkers & laddersrna reagents gene expressioncellular analysisepigeneticsprotein expression & purification technologiesprotein toolsglycobiologydna plasmidsbuffersstrainsdiscontinued new products special offers time for change monarch nucleic acid purification kits are optimized for maximum performance and minimal environmental impact . our unique column design offers elution in lower volumes and eliminates buffer retention resulting in high quality dna for downstream applications . monarch kits are available for plasmid miniprep gel extraction and reaction cleanup . learn more and request a sample tools & resources tools & resources overviewfaqsprotocolsselection chartstroubleshooting guidesusage guidelines tipsinteractive toolsvideo library popular toolsnebnext selectornebcloner nebiocalculator nebuilder assembly toolenzyme finderdouble digest findernebcutter rebase dna sequences and maps tooltm calculatorpcr selection toolpolbase neb 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