benches
sirna and mirna gene silencing from bench to bedside
p rna interference has become a key method in the suppression of gene expression and the development of therapeutic agents yet there is still the problem of delivery stability and the danger of off target effects such as the silencing of unwanted genes and activation of innate immunity . in sirna and mirna gene silencing from bench to bedside expert researchers explore the most recent advances in sirna design expression delivery in vivo imaging and methods to minimize sirna s unwanted effects and promote successful use in patients . as part of the highly successful methods in molecular biology series the chapters focus on their respective subjects with easy to use up to date information including several step by step laboratory protocols on topics such as new delivery formulations and strategies with promising applications in vitro and in vivo validated therapeutic target genes and components of mirna function biogenesis and interference with virus infection . p comprehensive and cutting edge sirna and mirna gene silencing from bench to bedside offers an excellent collection of chapters to aid all those with an interest in rnai gene regulation and new therapies .