Growth Experiment

Researcher

Susan M Stieve & Dennis P Stimart, U WI Horticulture

Description

This research concerned growth medium for cloning plants (Stieve, Stimart and Yandell 1992). Plant tissue (a small part of a leaf) was placed in a Petri dish which had a `food preparation' on it. The dish was sealed and placed in a controlled chamber with adequate light and proper temperature. The `food preparation', or medium, was designed to encourage growth of new plant shoots from the tissue. The response of interest here was the number of `adventitious shoots' (advplt), or new plants growing out of the tissue. Basically, the more adventitious shoots produced, the better the growing media. The adventitious plants can be separated and grown into numerous individuals for greenhouse cultivation, saving tremendous space and cost for nurseries and florists. The key question is: what is the best growing condition for the shoots? There were 12 factor combinations (trt) of interest, coming from a two-factor layout with added control (BHTA, trt = 20). One factor combination was missing; the plant material seemed to be burned by high levels of both chemicals together.

Reference

Stieve SM, Stimart DP and Yandell BS (1992) `Heritable tissue culture induced variation in Zinnia Marylandica', Euphytica 64, 81-89.

Measurements

growth.dat

trt ba tdz code advplt

SAS & S-Plus Data Analysis

growth.sas

growth.prt

growth.s

Book Figures

growtest.s

D:11.1 Growth interaction plots with a missing cell

growdat.s

E:13.2 Growth residual plots with zeros