To infer the impact of genomic architecture on the function, multi-omics data, such as ChIP-seq, ATAC-seq, and RNA-seq, need to be leveraged. I have formed multiple collaboration projects with Prof. Emery H. Bresnick lab from UW-Madison blood research program to reveals GATA-1/Heme regulation mechanism in controlling hemoglobin synthesis and erythrocyte development (Liao et al. 2018. In preparation). With the goal of uncovering GATA-1/Heme-dependent chromatin targeting, we quantified the individual and joint regulation effect of GATA-1 and Heme on DNA accessibility and gene expression. We also investigated the impact of single nucleotide mutation in the Ets motif of GATA2 enhancer on its function to control hematopoiesis through a comprehensive transcriptomic differential analysis (Tanimura et al. 2018. Developmental Cell). Ongoing collaborative projects are benefiting me with precious opportunity to get access to fresh multi-omics data conducted under the same experimental conditions. Beyond that, Encyclopedia of DNA Elements (ENCODE) project and 4D Nucleome program provide an unprecedented resource for 3D genome structure data across species, cell lines and tissues.
